Reza Alizadeh-Navaei; Majid Saeedi; Reza Valadan; Fatemeh Roozbeh; Omolbanin Amjadi; Ehsan Zaboli; Mahmood Moosazadeh; Hossein Ranjbaran; Zinab Qazizadeh; Ali Akbari-Malekshah; Touraj Assadi; Versa Omrani-Nava; Akbar Hedayatizadeh-Omran
Volume 22, Issue 7 , 2020
Abstract
Background: Coronavirus is a single-stranded RNA virus, causing an epidemic of pneumonia and acute respiratory distress syn- drome (ARDS) worldwide in late 2019.
Objectives: In addition to the clinical symptoms, laboratory diagnosis can greatly help diagnose the diseases; therefore, this study aimed ...
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Background: Coronavirus is a single-stranded RNA virus, causing an epidemic of pneumonia and acute respiratory distress syn- drome (ARDS) worldwide in late 2019.
Objectives: In addition to the clinical symptoms, laboratory diagnosis can greatly help diagnose the diseases; therefore, this study aimed to analyze laboratory parameters in patients with COVID-19.
Methods: In this cross-sectional study, the laboratory data of 2563 patients with COVID-19 admitted to hospitals affiliated with Mazandaran University of Medical Sciences were extracted from hospital information systems (HIS). The data were recorded on Excel and analyzed through t-test, chi-squared, and Fishers exact tests in SPSS 19 at the significance level of P < 0.05.
Results: Out of 2563 patients with a mean age of 55.1 ± 16.7 years, 1409 (55%) were male, and 1154 (45%) were female with a mean age of 55.7 ± 16.8, and 54.3 ± 16.6 years, respectively. As the most frequent clinical findings, ESR, CRP, and LDH were increased by 83.5%, 71%, and 69.3% of the patients, respectively. Other research findings included lymphopenia, disturbed INR, abnormal SGOT, abnormal alkaline phosphatase (ALP), and increased lactate dehydrogenase (LDH), which were significantly higher in men than in women and was different between age groups.
Conclusions: Conducting simple, convenient, and inexpensive laboratory tests can be helpful in the diagnosis of COVID-19.
Hossein Ranjbaran; Saeid Abediankenari; Marzei Momeninezhad Amiri
Volume 20, Issue 3 , 2018, Pages 1-7
Abstract
Background: With regards to the high potential of medicinal plants in the production of biopharmaceuticals, one can rely on the promising prospect of insulin production via plant resources. Objectives: This study was conducted with the aim of using plant extract for insulin-producing cells. Methods: ...
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Background: With regards to the high potential of medicinal plants in the production of biopharmaceuticals, one can rely on the promising prospect of insulin production via plant resources. Objectives: This study was conducted with the aim of using plant extract for insulin-producing cells. Methods: This was a quasi-experimental study. In this study, critical case sampling was used. Six samples were gathered from the umbilical cord (Wharton’s jelly) at Imam hospital, after successful isolation of mesenchymal stem cells. Initially, Nigella sativa seeds extraction was performed to prepare the extract for cellular differentiation. Next, dithizone (DTZ) staining was used to evaluate insulin production and insulin level was examined by the enzyme linked immunosorbent assay (ELISA) kit. All data was analyzed with the SPSS version 16 software using independent sample t-test. Results: The mean of the amount of insulin secretion was 92.33 ± 5.13 for the intervention and 0.33 ± 0.15 for the control group. The results showed that there was a significant difference in the average insulin in the culture obtained from Nigella sativa seeds between control and intervention groups (P = 0.001). In addition, via the ELISA kit and specific dithizone staining, insulin-producing cells were proven. Conclusions: In this regard, it could be concluded that the extract of Nigella sativa seeds was capable in the induction of differenti- ation of mesenchymal stem cells derived from Wharton’s jelly to IPCs.
